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Flow cytometry is an excellent way to measure DNA content because of its high sensitivity high precision acquisition of cell by cell total fluorescence, and of course DNA content analysis was the original fluorescent application of flow cytometry looking for an aneuploidy in cancer specimens. However, now-a-days the most common reason for measuring DNA content is because it gives us a very nice way to profile the position of cells within the cell cycle. Let's explore the cell cycle analysis plot in Kaluza Analysis Software together with Dr. David Onion.
Step-by-step instructions for generating a compensation matrix in CytExpert for CytoFLEX, the acquisition and analysis software used in the CytoFLEX Flow Cytometry platform.