CD34 Antibodies

The CD34 antigen is a monomeric transmembrane phosphoglycoprotein of about 110 kDa with two distinct extracellular domains. The membrane proximal domain, of about 110 amino acids, probably adopts a globular conformation. The NH2-terminal domain, of about 140 amino acids, is heavily glycosylated with both N-linked glycans and sialylated O-linked carbohydrates and probably exhibits an extended rod-like structure typical of mucin-like glycoproteins. The CD34 antigen is expressed on hematopoietic progenitor cells of all lineages as well as the most primitive pluripotential stem cells. CD34 antigen expression is highest on the most primitive stem cells and is gradually lost as lineage committed progenitors differentiate. The CD34 antigen is also present on capillary endothelial cells and on bone marrow stromal cells. Variations of glycosylation are thought to occur during normal hematopoiesis depending on lineage commitment and the level of cellular maturation.

Clone: Immu133 Isotype: IgG1 Mouse

Epitope Class I: Immu133 monoclonal antibody detects sialic acid-dependent class I epitope.

Clone: QBEnd10 Isotype: IgG1 Mouse

Epitope Class II: QBEnd10 reacts with early normal hematopoietic progenitor cells, and with vascular endothelial cells.

Clone: 581 Isotype: IgG1 Mouse

Epitope Class III: Clone 581 recognizes a sialidase / glycoprotease / chymopapain insensitive class III epitope.

Clone: QBEnd10 + Immu133 + Immu409 Isotype: IgG Mouse

The CD34 Pool Kit (IM1459U) includes two vials. The one labeled CD34 Pool-PE contains a mixture of QBEnd10, Immu133 and Immu409. The one labeled Isotypic Control IgG(1+2a)-PE is its matching negative control. As QBEnd10 and Immu133 are of the mouse IgG1 isotype and Immu409 of the IgG2a isotype, this control is a mixture of two R-phycoerythrin-conjugated irrelevant mouse monoclonal immunoglobulins of these isotypes. Each isotype concentration in the control matches the total concentration of each isotype in the CD34 Pool.

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