Microbioreactor Publications

Year Title Journal Authors Link
 2022 Developing a fluorometric urease activity microplate assay suitable for automated microbioreactor experimentsr  Frontiers Frédéric Lapierre, Isabel Bolz, Jochen Büchs, Robert Huber


 2022 Isolation and structural characterization of exopolysaccharide from marine Bacillus sp. and its optimization by Microbioreactor  Science Direct Vasudev S. Wagh, Madhukar S.Said, Jagadish S. Bennale, Syed G.Dastager


 2022 Therapeutic enzyme engineering using a generative neural network Scientific Reports Andrew Giessel, Athanasios Dousis, Kanchana Ravichandran, Kevin Smith, Sreyoshi Sur, Iain McFadyen, Wei Zheng & Stuart Licht https://doi.org/10.1038/s41598-022-05195-x
2021 Microbioreactor-assisted cultivation workflows for time-efficient phenotyping of protein producing Aspergillus niger in batch and fed-batch mode Biotechnoly Progress Roman Jansen, Holger Morschett, Dennis Hasenklever, Matthias Moch, Wolfgang Wiechert, Marco Oldiges https://doi.org/10.1002/btpr.3144
2021 A fully automated pipeline for the dynamic at‐line morphology analysis of microscale Aspergillus cultivation Fungal Biol Biotechnol Roman Jansen, Kira Küsters, Holger Morschett, Wolfgang Wiechert and Marco Oldiges https://doi.org/10.1186/s40694-021-00109-4
2021 High‑throughput microbioreactor provides a capable tool for early stage bioprocess development Scientific Reports Mathias Fink, Monika Cserjan‑Puschmann, Daniela Reinisch and Gerald Striedner https://doi.org/10.1038/s41598-021-81633-6
2021 Butanol production from lignocellulosic sugars by Clostridium beijerinckii in microbioreactors Biotechnology for Biofuels Cansu Birgen, Kristin F. Degnes, Sidsel Markussen, Alexander Wentzel and Håvard Sletta https://doi.org/10.1186/s13068-021-01886-1
2020 Revealing nutritional requirements of MICP‑relevant Sporosarcina pasteurii DSM33 for growth improvement in chemically defined and complex media Scientific Reports Frédéric M. Lapierre, Jakob Schmid, Benjamin Ederer, Nina Ihling, Jochen Büchs & Robert Huber https://doi.org/10.1038/s41598-020-79904-9
2020 Machine learning for metabolic engineering: A review Metabolic Engineering Christopher E. Lawson, Jose Manuel Martí, Tijana Radivojevic, Sai Vamshi R. Jonnalagadda, Reinhard Gentz, Nathan J. Hillson, Sean Peisert, Joonhoon Kim, Blake A. Simmons, Christopher J. Petzold, Steven W. Singer, Aindrila Mukhopadhyay, Deepti Tanjore, Joshua G. Dunn, Hector Garcia Martin https://doi.org/10.1016/j.ymben.2020.10.005
2020 The GATA Transcription Factor Gaf1 Represses tRNAs, Inhibits Growth, and Extends Chronological Lifespan Downstream of Fission Yeast TORC1 Cell Reports María Rodríguez-López, Suam Gonzalez, Olivia Hillson, Victor A. Tallada, Jürg Bähler, Charalampos Rallis https://doi.org/10.1016/j.celrep.2020.02.058
2020 Small scale screening of yeast strains enables high-throughput evaluation of performance in lignocellulose hydrolysates Bioresource Technology Reports Marlous van Dijk, Ignis Trollmann, Margarete Alice Fontes Saraiva, Rogelio Lopes Brandão, Lisbeth Olsson, Yvonne Nygård https://doi.org/10.1016/j.biteb.2020.100532
2020 Adaptive laboratory evolution of Pseudomonas putida KT2440 improves p-coumaric and ferulic acid catabolism and tolerance Metabolic Engineering Communications Elsayed T. Mohamed, Allison Z. Werner, Davinia Salvachúa, Christine A. Singer, Kiki Szostkiewicz, Manuel Rafael Jiménez-Díaz, Thomas Eng, Mohammad S. Radi, Blake A. Simmons, Aindrila Mukhopadhyay, Markus J. Herrgård, Steven W. Singer,Gregg T. Beckham, Adam M. Feist https://doi.org/10.1016/j.mec.2020.e00143
2020 Formulating bacterial endophyte: Pre-conditioning of cells and the encapsulation in amidated pectin beads Biotechnology Reports Mauricio Cruz Barrera, Desiree Jakobs-Schoenwandt, Martha Isabel Gómez, Juan Serrato, Silke Ruppel, Anant V. Patel https://doi.org/10.1016/j.btre.2020.e00463
2020 Genome Sequence and Characterization of Five Bacteriophages Infecting Streptomyces coelicolor and Streptomyces venezuelae: Alderaan, Coruscant, Dagobah, Endor1 and Endor2 Viruses Aël Hardy, Vikas Sharma, Larissa Kever and Julia Frunzke https://doi.org/10.3390/v12101065
2020 A unified design allows fine-tuning of biosensor parameters and application across bacterial species Metabolic Engineering Communications Christiane Katharina Sonntag, Lion Konstantin Flachbart, Celine Maass, Michael Vogt, Jan Marienhagen https://doi.org/10.1016/j.mec.2020.e00150
2020 Multidimensional engineering of Saccharomyces cerevisiae for efficient synthesis of medium-chain fatty acids Nature Catalysis Zhiwei Zhu, Yating Hu, Paulo Gonçalves Teixeira, Rui Pereira, Yun Chen, Verena Siewers and Jens Nielsen https://doi.org/10.1038/s41929-019-0409-1
2019 Development of a novel, robust and cost-efficient process for valorizing dairy waste exemplified by ethanol production Microb Cell Fact. Jing Shen, Jun Chen, Peter Ruhdal Jensen and Christian Solem https://doi.org/10.1186/s12934-019-1091-3
2019 Butanol production from lignocellulosic biomass: revisiting fermentation performance indicators with exploratory data analysis Biotechnol Biofuels Cansu Birgen, Peter Dürre, Heinz A. Preisig and Alexander Wentzel https://doi.org/10.1186/s13068-019-1508-6
2019 Less Sacrifice, More Insight: Repeated Low-Volume Sampling of Microbioreactor Cultivations Enables Accelerated Deep Phenotyping of Microbial Strain Libraries Biotechnol J. Johannes Hemmerich, Niklas Tenhaef, Carmen Steffens, Jannick Kappelmann, Marc Weiske, Sebastian J Reich, Wolfgang Wiechert, Marco Oldiges, Stephan Noack https://doi.org/10.1002/biot.201800428
2019 Glucose-containing polymer rings enable fed-batch operation in microtiter plates with parallel online measurement of scattered light, fluorescence, dissolved oxygen tension, and pH Biotechnol Bioeng. Tobias Habicher, Vroni Czotscher, Tobias Klein, Andreas Daub, Timm Keil, Jochen Büchs https://doi.org/10.1002/bit.27077
2019 A FRET-based biosensor for the quantification of glucose in culture supernatants of mL scale microbial cultivations Microb Cell Fact. Julia Otten, Niklas Tenhaef, Roman P Jansen, Johannes Döbber, Lisa Jungbluth, Stephan Noack, Marco Oldiges, Wolfgang Wiechert, Martina Pohl https://doi.org/10.1186/s12934-019-1193-y
2019 Function of L-Pipecolic Acid as Compatible Solute in Corynebacterium glutamicum as Basis for Its Production Under Hyperosmolar Conditions Front Microbiol. Fernando Pérez-García, Luciana F. Brito, and Volker F. Wendisch https://doi.org/10.3389/fmicb.2019.00340
2019 The two model organisms E. coli and H. polymorpha were cultivated in the fed-batch microtiter plate to investigate the general applicability for microbial systems. J Biol Eng. T. Keil, B. Dittrich, C. Lattermann, T. Habicher, and J. Büchs https://doi.org/10.1186/s13036-019-0147-6
2019 Alone at last! - Heterologous expression of a single gene is sufficient for establishing the five-step Weimberg pathway in Corynebacterium glutamicum Metab Eng Commun. Christian Brüsseler, Anja Späth, Sascha Sokolowsky, and Jan Marienhagen https://doi.org/10.1016/j.mec.2019.e00090
2019 Parallelized microscale fed-batch cultivation in online-monitored microtiter plates: implications of media composition and feed strategies for process design and performance Journal of Industrial Microbiology & Biotechnology Holger Morschett, Roman Jansen, Christian Neuendorf, Matthias Moch, Wolfgang Wiechert, Marco Oldiges https://doi.org/10.1007/s10295-019-02243-w
2019 A closer look at Aspergillus: online monitoring via scattered light enables reproducible phenotyping Fungal Biology and Biotechnology Roman P. Jansen, Carina Beuck, Matthias Moch, Bianca Klein, Kira Küsters, Holger Morschett, Wolfgang Wiechert and Marco Oldiges https://doi.org/10.1186/s40694-019-0073-x
2019 Innovative microscale workflow from fungi cultures to Cell Wall-Degrading Enzyme screening Microbial Biotechnology, Roxane Raulo, Egon Heuson, Ali Siah, Vincent Phalip and Renato Froidevaux https://doi.org/10.1111/1751-7915.13405
2019 Engineering Robust Production Microbes for Large-Scale Cultivation Trends in Microbiology Maren Wehrs, Deepti Tanjore, Thomas Eng, Jeff Lievense, Todd R. Pray, and Aindrila Mukhopadhyay https://doi.org/10.1016/j.tim.2019.01.006
2019 Engineered microbial biofuel production and recovery under supercritical carbon dioxide Nature Communications J. T. Boock, A. J. E. Freedman, G. A. Tompsett, S. K. Muse, A. J. Allen, L. A. Jackson, B. Castro-Dominguez, M. T. Timko, K. L. J. Prather & J. R. Thompson https://doi.org/10.1038/s41467-019-08486-6
2018 Metabolic engineering of Corynebacterium glutamicum for the production of cis, cis-muconic acid from lignin Microb Cell Fact. Judith Becker, Martin Kuhl, Michael Kohlstedt, Sören Starck, and Christoph Wittmann https://doi.org/10.1186/s12934-018-0963-2
2018 Fermentative Production of N-Methylglutamate From Glycerol by Recombinant Pseudomonas putida Front Bioeng Biotechnol. Melanie Mindt, Tatjana Walter, Joe Max Risse, and Volker F. Wendisch https://doi.org/10.3389/fbioe.2018.00159
2018 Germination and Growth Analysis of Streptomyces lividans at the Single-Cell Level Under Varying Medium Compositions Front Microbiol. Joachim Koepff, Christian Carsten Sachs, Wolfgang Wiechert, Dietrich Kohlheyer, Katharina Nöh, Marco Oldiges, and Alexander Grünberger https://doi.org/10.3389/fmicb.2018.02680
2018 One-step process for production of N-methylated amino acids from sugars and methylamine using recombinant Corynebacterium glutamicum as biocatalyst Sci Rep. Melanie Mindt, Joe Max Risse, Hendrik Gruß, Norbert Sewald, Bernhard J. Eikmanns, and Volker F. Wendisch https://doi.org/10.1186/s12934-018-0968-x
2018 Production of Food and Feed Additives From Non-food-competing Feedstocks: Valorizing N-acetylmuramic Acid for Amino Acid and Carotenoid Fermentation With Corynebacterium glutamicum Front Microbiol. Elvira Sgobba, Luisa Blöbaum, and Volker F. Wendisch https://doi.org/10.3389/fmicb.2018.02046
2018 High‑throughput optimization of medium components and culture conditions for the efficient production of a lipopeptide pseudofactin by Pseudomonas fluorescens BD5 Biniarz et al. Microb Cell Fact Piotr Biniarz, François Coutte, Frédérique Gancel and Marcin Łukaszewicz https://doi.org/10.1186/s12934-018-0968-x
2018 A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineered Corynebacterium glutamicum Green Chem. Christina Maria Rohles, Lars Gläser, Michael Kohlstedt, Gideon Gießelmann, Samuel Pearson, Aránzazu del Campo, Judith Beckera and Christoph Wittmann https://doi.org/10.1039/C8GC01901K
2018 Microbioreactor Systems for Accelerated Bioprocess Development Biotechnol. J. Johannes Hemmerich, Stephan Noack, Wolfgang Wiechert, and Marco Oldiges https://doi.org/10.1002/biot.201700141
2018 Glucose-Dependent Promoters for Dynamic Regulation of Metabolic Pathways Frontiers in Bioengineering and Biotechnology, Maury Jérôme, Kannan Soumya, Jensen Niels B., Öberg Fredrik K., Kildegaard Kanchana R., Forster Jochen, Nielsen Jens, Workman Christopher T., Borodina Irina https://doi.org/10.3389/fbioe.2018.00063
2018 A bio-based route to the carbon-5 chemical glutaric acid and to bionylon-6,5 using metabolically engineered Corynebacterium glutamicum Green Chem. Christina Maria Rohles, Lars Gläser, Michael Kohlstedt, Gideon Gießelmann, Samuel Pearson, Aránzazu del Campo, Judith Beckera and Christoph Wittmann https://doi.org/10.1039/C8GC01901K
2018 An optogenetic toolbox of LOV-based photosensitizers for light-driven killing of bacteria Scientific Reports S. Endres, M. Wingen, J. Torra, R. Ruiz-González, T. Polen, G. Bosio, N. L. Bitzenhofer, F. Hilgers, T. Gensch, S. Nonell, K.-E. Jaeger and T. Drepper https://doi.org/10.1038/s41598-018-33291-4
2018 Layered dynamic regulation for improving metabolic pathway productivity in Escherichia coli Proceedings of the National Academy of Sciences Stephanie J. Doong, Apoorv Gupta and Kristala L. J. Prather https://doi.org/10.1073/pnas.1716920115
2018 Doing More With Less: Using High-Throughput and Parallel Experimental Systems to Enhance Learning IATED Digital Library V. Xiao, J. Abdul-Raheem, J.F. Hamel https://library.iated.org/view/XIAO2018DOI
2017 On the catabolism of amino acids in the yeast Dekkera bruxellensis and the implications for industrial fermentation processes Wiley Online Library Denise Castro Parente, Danielli Batista Bezerra Cajueiro, Irina Charlot Peña Moreno, Fernanda Cristina Bezerra Leite, Will De Barros Pita, Marcos Antonio De Morais Jr https://doi.org/10.1002/yea.3290
2017 Managing the SOS Response for Enhanced CRISPR-Cas-Based Recombineering in E. coli through Transient Inhibition of Host RecA Activity ACS Synth. Biol. Eirik Adim Moreb, Benjamin Hoover, Adam Yaseen, Nisakorn Valyasevi, Zoe Roecker, Romel Menacho-Melgar, and Michael D. Lynch https://doi.org/10.1021/acssynbio.7b00174
2017 The Experiment Data Depot: A Web-Based Software Tool for Biological Experimental Data Storage, Sharing, and Visualization ACS Synth. Biol. William C. Morrell, Garrett W. Birkel, Mark Forrer, Teresa Lopez, Tyler W. H. Backman, Michael Dussault, Christopher J. Petzold et al. https://doi.org/10.1021/acssynbio.7b00204
2017 Identification of genes affecting alginate biosynthesis in Pseudomonas fluorescens by screening a transposon insertion library BMC genomics Ertesvåg, H., Sletta, H., Senneset, M., Sun, Y. Q., Klinkenberg, G., Konradsen, T. A., Ellingsen, T. E., and Valla, S. https://doi.org/10.1186/s12864-016-3467-7
2017 CRISPR/Cas9-based genome editing for simultaneous interference with gene expression and protein stability Nucleic Acids Research Virginia Martinez, Ida Lauritsen, Tonja Hobel, Songyuan Li, Alex Toftgaard Nielsen and Morten H. H. Nørholm https://doi.org/10.1093/nar/gkx797
2017 Fast and reliable strain characterization of Streptomyces lividans through micro-scale cultivation Biotechnol Bioeng. Koepff J., Keller M., Tsolis K.C., Busche T., Rückert C., Hamed M.B., Anné J., Kalinowski J., Wiechert W., Economou A., Oldiges M. https://doi.org/10.1002/bit.26321
2017 Prediction of Escherichia coli expression performance in microtiter plates by analyzing only the temporal development of scattered light during culture Journal of Biological Engineering Ladner T., Mühlmann M., Schulte A., Wandrey G. and Büchs J. https://doi.org/10.1186/s13036-017-0064-5
2017 Regulation of solvent tolerance in Pseudomonas putidaS12 mediated by mobile elements Microbial Biotechnology Hosseini R., Kuepper J., Koebbing S., Blank L.M., Wierckx N. and de Winde J.h https://doi.org/10.1111/1751-7915.12495
2017 Dynamic regulation of metabolic flux in engineered bacteria using a pathway-independent quorum-sensing circuit Nature Biotechnology Gupta A., Brockman Reizman I., Reisch C. and Prather K. https://doi.org/10.1038/nbt.3796
2017 Cellulolytic RoboLector - towards an automated high-throughput screening platform for recombinant cellulase expression Journal of Biological Engineering Mühlmann M, Kunze M., Ribeiro J., Geinitz B., Lehmann C., Schwaneberg U., Commandeur U. and Büchs J. https://doi.org/10.1186/s13036-016-0043-2
2017 Comparative evaluation of phototrophic microtiter plate cultivation against laboratory-scale photobioreactors Bioprocess and Biosystems Engineering Morschett H., Schiprowski D., Rohde J., et al. https://doi.org/10.1007/s00449-016-1731-5
2017 The Experiment Data Depot: a web-based software tool for biological experimental data storage, sharing, and visualization ACS Synth. Biol. Morrell W., Birkel G., Forrer M., Lopez T., Backman T., Dussault M., Petzold C.J., et al. https://doi.org/10.1021/acssynbio.7b00204
2017 Beschleunigte Bioprozessentwicklung im automatisierten Mikromaßstab Biospektrum Holger Morschett, Stephan Noack, Marco Oldiges https://doi.org/10.1007/s12268-017-0838-9
2016 Zymomonas mobilis as a model system for production of biofuels and biochemicals Microbial Biotechnology Shihui Yang Qiang Fei Yaoping Zhang Lydia M. Contreras Sagar M. Utturkar Steven D. Brown Michael E. Himmel Min Zhang https://doi.org/10.1111/1751-7915.12408
2016 Quasi-continuous parallel online scattered light, fluorescence and dissolved oxygen tension measurement combined with monitoring of the oxygen transfer rate in each well of a shaken microtiter plate Microbial Cell Factories Ladner T., Held M., Flitsch D., Beckers M. and Büchs J. https://doi.org/10.1186/s12934-016-0608-2
2016 High-throughput strategies for the discovery and engineering of enzymes for biocatalysis Bioprocess and Biosystems Engineering Jacques P., Béchet M., Bigan M. et al. https://doi.org/10.1007/s00449-016-1690-x
2016 High-throughput fermentation screening for the yeast Yarrowia lipolytica with real-time monitoring of biomass and lipid production Microbial Cell Factories Back A., Rossignol T., Krier F., Nicaud J.-M. and Dhulster P. https://doi.org/10.1186/s12934-016-0546-z
2016 An improvement of surfactin production by B. subtilis BBG131 using design of experiments in microbioreactors and continuous process in bubbleless membrane bioreactor Bioresource Technology Motta Dos Santos L. F., Coutte F., Ravallec R., Dhulster P., Tournier-Couturier L., Jacques P. https://doi.org/10.1016/j.biortech.2016.07.053
2016 Identification of Essential Genetic Baculoviral Elements for Recombinant Protein Expression by Transactivation in Sf21 Insect Cells PLoS ONE Bleckmann M, Schürig M, Chen F-F, Yen Z-Z, Lindemann N, Meyer S, et al. https://doi.org/10.1371/journal.pone.0149424
2016 Fast plasmid based protein expression analysis in insect cells using an automated SplitGFP screen Biotechnology and Bioengineering Bleckmann, M., Schmelz, S., Schinkowski, C., Scrima, A. and van den Heuvel, J. https://doi.org/10.1002/bit.25956
2015 Improvement of glucaric acid production in E. coli via dynamic control of metabolic fluxes Metabolic Engineering Communications Irene M. Brockman Reizman, Andrew R. Stenger, Chris R. Reisch, Apoorv Gupta, Neal C. Connors, Kristala L.J. Prather https://doi.org/10.1016/j.meteno.2015.09.002
2015 Improvement of glucaric acid production in E. coli via dynamic control of metabolic fluxes Metabolic Engineering Communications Irene M. Brockman Reizman, Andrew R. Stenger, Chris R. Reisch, Apoorv Gupta, Neal C. Connors, Kristala L. J. Prather https://doi.org/10.1016/j.meteno.2015.09.002
2015 Exploring the role of sigma factor gene expression on production by Corynebacterium glutamicum: sigma factor H and FMN as example Frontiers in Microbiology Taniguchi H. and Wendisch V. F. https://doi.org/10.3389/fmicb.2015.00740
2015 A TatABC-Type Tat Translocase Is Required for Unimpaired Aerobic Growth of Corynebacterium glutamicum ATCC13032 PLoS ONE Oertel D., Schmitz S., Freudl R. https://doi.org/10.1371/journal.pone.0123413
2015 Genomic Analysis and Isolation of RNA Polymerase II Dependent Promoters from Spodoptera frugiperda PLoS ONE Bleckmann M., Fritz M. H.-Y., Bhuju S., Jarek M., Schürig M., Geffers R., Benes V., Besir H., van den Heuvel J. https://doi.org/10.1371/journal.pone.0132898
2015 Bioprocess automation on a Mini Pilot Plant enables fast quantitative microbial phenotyping Microbial Cell Factories Unthan S.,Radek A.,Wiechert W.,Oldiges M. and Noack S. https://doi.org/10.1186/s12934-015-0216-6
2015 Fed-batch like cultivation in a micro-bioreactor: screening conditions relevant for Escherichia coli based production processes SpringerPlus Toeroek C., Cserjan-Puschmann M., Bayer K. and Striedner G https://doi.org/10.1186/s40064-015-1313-z
2015 Modeling leucine's metabolic pathway and knockout prediction improving the production of surfactin, a biosurfactant from Bacillus subtilis Biotechnology Journal Coutte F., Niehren J., Dhali D., John M., Versari C. and Jacques Ph. https://doi.org/10.1002/biot.201400541
2015 High‐throughput micro‐scale cultivations and chromatography modeling: Powerful tools for integrated process development Biotechnology and Bioengineering Baumann P., Hahn T. and Hubbuch J. https://doi.org/10.1002/bit.25630
2015 Parallel use of shake flask and microtiter plate online measuring devices (RAMOS and BioLector) reduces the number of experiments in laboratory-scale stirred tank bioreactors Journal of Biological Engineering Wewetzer S. J., Kunze M., Ladner T., Luchterhand B., Roth S., Rahmen N., Kloß R., Costa e Silva A., Regestein L. and Büchs J. https://doi.org/10.1186/s13036-015-0005-0
2015 Chassis organism from Corynebacterium glutamicum - a top-down approach to identify and delete irrelevant gene clusters Biotechnol J. Unthan S., Baumgart M., Radek A., Herbst M., Siebert D., Brühl N., Bartsch A., Bott M., Wiechert W., Marin K., Hans S., Krämer R., Seibold G., Frunzke J., Kalinowski J., Rückert C., Wendisch V.F., Noack S. https://doi.org/10.1002/biot.201400041
2014 Combinatorial Strategies for Improving Multiple-Stress Resistance in Industrially Relevant Escherichia coli Strains American Society for Microbiology Rebecca M. Lennen, Markus J. Herrgård https://doi.org/10.1128/AEM.01542-14
2014 Using Synthetic Biological Parts and Microbioreactors to Explore the Protein Expression Characteristics of Escherichia coli ACS Synthetic Biology Gorochowski T. E., van den Berg E., Kerkman R., Roubos J. A. and Bovenberg R. A. L. https://doi.org/10.1021/sb4001245
2014 Global analysis of protein structural changes in complex proteomes Nature Biotechnology Feng Y., De Franceschi G., Kahraman A., Soste M., Melnik A., Boersema P. J., Polverino de Laureto P., Nikolaev Y., Oliveira A. P. and Picotti P. https://doi.org/10.1038/nbt.2999
2014 Chassis organism from Corynebacterium glutamicum - a top-down approach to identify and delete irrelevant gene clusters Biotechnology Journal Unthan S., Baumgart M., Radek A., Herbst M., Siebert D., Brühl N., Bartsch A., Bott M., Wiechert W., Marin K., Hans S., Krämer R., Seibold G., Frunzke J., Kalinowski J., Rückert C., Wendisch V. F. and Noack S. https://doi.org/10.1002/biot.201400041
2014 Rapid assessment of oxygen transfer impact for Corynebacterium glutamicum Bioprocess and Biosystems Engineering Käß F., Prasad A., Tillack J., Moch M., Giese H., Büchs J., Wiechert W. and Oldiges M. https://doi.org/10.1007/s00449-014-1234-1
2014 Comprehensive clone screening and evaluation of fed-batch strategies in a microbioreactor and lab scale stirred tank bioreactor system: application on Pichia pastoris producing Rhizopus oryzae Lipase Microbial Cell Factories Hemmerich J., Adelantado N., Barrigón J.M., Ponte X., Hörmann A., Ferrer P., Kensy F. and Valero F. https://doi.org/10.1186/1475-2859-13-36
2014 SoxR as a Single-Cell Biosensor for NADPH-Consuming Enzymes in Escherichia coli ACS Synthetic Biology Siedler S., Schendzielorz G., Binder S., Eggeling L., Bringer S. and Bott M. https://doi.org/10.1021/sb400110j
2014 Taking Control over Control: Use of Product Sensing in Single Cells to Remove Flux Control at Key Enzymes in Biosynthesis Pathways ACS Synthetic Biology Schendzielorz G., Dippong M., Grünberger A., Kohlheyer D., Yoshida A., Binder S., Nishiyama C., Nishiyama M., Bott M. and Eggeling L. https://doi.org/10.1021/sb400059y
2014 Beyond Growth Rate 0.6: What Drives Corynebacterium glutamicum to Higher Growth Rates in Defined Medium Biotechnology and Bioengineering Unthan S., Grünberger A., van Ooyen J., Gätgens J., Heinrich J., Paczia N., Wiechert W., Kohlheyer D. and Noack S. https://doi.org/10.1002/bit.25103
2014 Application of a Genetically Encoded Biosensor for Live Cell Imaging of L-Valine Production in Pyruvate Dehydrogenase Complex-Deficient Corynebacterium glutamicum Strains PLoS ONE Mustafi N., Grünberger A., Mahr R., Helfrich S., Nöh K., Blombach B., Kohlheyer D. and Frunzke J. https://doi.org/10.1371/journal.pone.0085731
2014 Analysis of SOS-Induced Spontaneous Prophage Induction in Corynebacterium glutamicum at the Single-Cell Level Journal of Bacteriology Nanda A. M., Heyer A., Krämer C., Grünberger A., Kohlheyer D. and Frunzke J. https://doi.org/10.1128/JB.01018-13
2013 Transposon Mutagenesis Identified Chromosomal and Plasmid Genes Essential for Adaptation of the Marine Bacterium Dinoroseobacter shibae to Anaerobic Conditions Journal of Bacteriology Matthias Ebert, Sebastian Laaß, Melanie Burghartz, Jörn Petersen, Sebastian Koßmehl, Lars Wöhlbrand, Ralf Rabus, Christoph Wittmann, Petra Tielen, Dieter Jahna https://doi.org/10.1128/JB.00860-13
2013 IpsA, a novel LacI-type regulator, is required for inositol-derived lipid formation in Corynebacteria and Mycobacteria BMC Biology Baumgart M., Luder K., Grover S., Gätgens C., Besra G.S. and Frunzke J. https://doi.org/10.1186/1741-7007-11-122
2013 Complex Regulation of the Phosphoenolpyruvate Carboxykinase Gene pck and Characterization of Its GntR-Type Regulator IolR as a Repressor of myo-Inositol Utilization Genes in Corynebacterium glutamicum Journal of Bacteriology Klaffl S., Brocker M., Kalinowski J., Eikmanns B.J. and Bott M. https://doi.org/10.1128/JB.00265-13
2013 Systems metabolic engineering of Corynebacterium glutamicum for production of the chemical chaperone ectoine. Microbial Cell Factories Becker J., Schäfer R., Kohlstedt M., Harder B.J., Borchert N.S., Stöveken N., Bremer E. and Wittmann C. https://doi.org/10.1186/1475-2859-12-110
2013 Visualization of Imbalances in Sulfur Assimilation and Synthesis of Sulfur-Containing Amino Acids at the Single-Cell Level Applied and Environmental Microbiology Hoffmann K., Grünberger A., Lausberg F., Bott M. and Eggeling L. https://doi.org/10.1128/AEM.01804-13
2013 Construction of a Prophage-Free Variant of Corynebacterium glutamicum ATCC 13032 for Use as a Platform Strain for Basic Research and Industrial Biotechnology. Applied and Environmental Microbiology Baumgart M., Unthan S., Rückert C., Sivalingam J., Grünbergerr A., Kalinowski J., Bott M., Noack S. and Frunzke J. https://doi.org/10.1128/AEM.01634-13
2013 Ornithine cyclodeaminase-based proline production by Corynebacterium glutamicum Microbial Cell Factories Jensen J.V.K. and Wendisch V.F. https://doi.org/10.1186/1475-2859-12-63
2013 Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24 Biotechnology for Biofuels Hector R.E., Dien B.S., Cotta M.A. and Mertens J.A. https://doi.org/10.1186/1754-6834-6-84
2013 Recombineering in Corynebacterium glutamicum combined with optical nanosensors: a general strategy for fast producer strain generation Nucleic Acids Research Binder S., Siedler S., Marienhagen J., Bott M. and Eggeling L. https://doi.org/10.1093/nar/gkt312
2013 Destabilized eYFP variants for dynamic gene expression studies in Corynebacterium glutamicum Microbial Biotechnology Hentschel E., Will C., Mustafi N., Burkovski A., Rehm N. and Frunzke J. https://doi.org/10.1111/j.1751-7915.2012.00360.x
2013 Beyond growth rate 0.6: Corynebacterium glutamicum cultivated in highly diluted environments Biotechnology & Bioengineering Grünberger A., van Ooyen J., Paczia N., Rohe P., Schiendzielorz G., Eggeling L., Wiechert W., Kohlheyer D. and Noack S. https://doi.org/10.1002/bit.24616
2012 Fed-Batch Microbioreactor Platform for Scale Down and Analysis of a Plasmid DNA Production Process Biotechnology and Bioengineering Diana M. Bower, Kevin S. Lee, Rajeev J. Ram, Kristala L.J. Prather https://doi.org/10.1002/bit.24498
2012 Short-chain fluorescent tryptophan tags for on-line detection of functional recombinant proteins. BMC Biotechnology Siepert E.-M., Gartz E., Tur M.K., Delbrück H., Barth S. and Büchs J. https://doi.org/10.1186/1472-6750-12-65
2012 Quantification of proteome dynamics in Corynebacterium glutamicum by 15N-labeling and selected reaction monitoring Journal of Proteomics Voges R. and Noak S. https://doi.org/10.1016/j.jprot.2012.03.020
2012 Expression of recombinant staphylokinase in the methylotrophic yeast Hansenula polymorpha BMC Biotechnology Moussa M., Ibrahim M., El Ghazaly M., Rohde J., Gnoth S., Anton A., Kensy F. and Mueller F. https://doi.org/10.1186/1472-6750-12-96
2012 An automated workflow for enhancing microbial bioprocess optimization on a novel microbioreactor platform Microbial Cell Factories Rohe P., Venkanna D., Kleine B., Freudl R. and Oldiges M. https://doi.org/10.1186/1475-2859-11-144
2012 The development and application of a single-cell biosensor for the detection of L-methionine and branched-chain amino acids Metabolic Engineering Mustafi N., Grünberger A., Kohlheyer D., Bott M. and Frunzke J. https://doi.org/10.1016/j.ymben.2012.02.002
2012 A high-throughput approach to identify genomic variants of bacterial metabolite producers at the single-cell level Genome Biology Binder S., Schendzielorz G., Stabler N., Krumbach K., Hoffmann K., Bott M. and Eggeling L. https://doi.org/10.1186/gb-2012-13-5-r40
2012 Hochparallele Bioprozessentwicklung in geschüttelten MikrobioreaktorenHigh-Throughput Bioprocess Development in Shaken Microbioreactors Chemie Ingenieur Technik Wenk P., Hemmerich J., Müller C. and Kensy F. https://doi.org/10.1002/cite.201100206
2012 Real-time determination of intracellular oxygen in bacteria using a genetically encoded FRET-based biosensor BMC Biology Potzkei J., Kunze M., Drepper T., Gensch T., Jaeger K.-E. and Büchs J. https://doi.org/10.1186/1741-7007-10-28
2011 Growth independent rhamnolipid production from glucose using the non-pathogenic Pseudomonas putida KT2440 Microbial Cell Factories Wittgens A., Tiso T., Arndt T.T., Wenk P., Hemmerich J., Müller C., Wichmann R., Küpper B., Zwick M., Wilhelm S., Hausmann R., Syldatk C., Rosenau F. and Blank L.M. https://doi.org/10.1186/1475-2859-10-80
2010 Bioprocess control in Microscale: Scalable fermentations in disposable and user-friendly microfluidic systems Microbial Cell Factories Funke M., Buchenauer A., Mokwa W., Kluge S., Hein L., Müller C., Kensy F. and Büchs J. https://doi.org/10.1186/1475-2859-9-86
2010 Microfluidic BioLector - Microfluidic Bioprocess Control in Microtiter Plates Biotechnology & Bioengineering Funke M., Buchenauer A., Schnakenberg U., Mokwa W., Diederichs S., Mertens A., Müller C., Kensy F. and Büchs J. https://doi.org/10.1002/bit.22825
2010 Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters Applied and Environmental Microbiology Drepper T., Huber R. Heck A., Circolone F., Hillmer A.-K., Büchs J. and Jaeger K.-E. https://doi.org/10.1128/AEM.00701-10
2010 Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring BMC Biotechnology Huber R., Palmen T.G., Ryk N., Hillmer A.-K., Luft K., Kensy F. and Büchs J. https://doi.org/10.1186/1472-6750-10-22
2010 High-throughput screening of Hansenula polymorpha clones in the batch compared with the controlled-release fed-batch mode on a small scale FEMS Yeast Research Scheidle M., Jeude M., Dittrich B., Denter S., Kensy F., Suckow M., Klee D. and Büchs J. https://doi.org/10.1111/j.1567-1364.2009.00586.x
2010 Charakterisierung von Cellulasepräparationen mittels Streulicht Chemie Ingenieur Technik Wulfhorst H., Jäger G., Ellinidou E., Büchs J. and Spiess A. https://doi.org/10.1002/cite.200900126
2009 Scale-up from microtiter plate to laboratory fermenter: Evaluation by online monitoring techniques of growth and protein expression in Escherichia coli and Hansenula polymorpha fermentations Microbial Cell Factories Kensy F., Engelbrecht C. and Büchs J. https://doi.org/10.1186/1475-2859-8-68
2009 Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plates cultivations at phosphate limitation Biotechnol. Bioeng. Kottmeier K., Weber J., Müller M., Bley T. and Büchs J. https://doi.org/10.1002/bit.22410
2009 Validation of a high-throughput fermentation system based on online monitoring of biomass and fluorescence in continuously shaken microtiter plates Microbial Cell Factories Kensy F., Zang E., Faulhammer F., Tan R.-K. and Büchs J. https://doi.org/10.1186/1475-2859-8-31
2009 The baffled microtiter plate: Increased oxygen transfer and improved online monitoring in small scale fermentations Biotechnology & Bioengineering Funke M., Diederichs S., Kensy F., Müller C. and Büchs J. https://doi.org/10.1002/bit.22341
2009 Oxygen transfer phenomena in 48-well microtiter plates: Determination by optical monitoring of sulfite oxidation and verification by real-time measurement during microbial growth Biotechnol. Bioeng. Kensy F., Zimmermann H.F., Knabben I., Anderlei T., Trauthwein H., Dingerdissen U. and Büchs J. https://doi.org/10.1002/bit.20373

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