Advantages & Applications of the BioLector XT Microbioreactor

High-throughput microbioreactor enables real-time evaluation of biomass, fluorescence, pH, dissolved oxygen in the liquid phase (DO), and other key cultivation parameters for aerobes and anaerobes.

How microbioreactors for high-throughput cultivations accelerate your bioprocess development

Building on trusted BioLector Pro technology, the BioLector XT microbioreactor is based on a standard ANSI/SLAS (SBS) microtiter plate (MTP) format, and operates with online, pre-calibrated optical sensors. Disposable 48 well MTPs enable online measurement of biomass, fluorescence, pH and DO, while patented microfluidic technology supports simultaneous pH control and feeding. The optional microfluidic module eliminates manual liquid handling—no tubing or pipetting required, as everything is part of the gamma-radiated ready-to-use plates.

Scale-up is the bottleneck in bioprocess development. It takes a lot of time, drastically slowing down early-stage development and thus the path to the final product. From early screening experiments in shake flasks over microbial cultivation in smaller fermentors to production scale, your processes can take days or weeks. You can, of course, reduce the time by using multiple fermentors simultaneously. But this is limited by your available space, human resources and budget. Imagine how costly it would be to run 32, or even 48, parallel fermentations in sizable fermentors …

And now imagine if you could achieve such a throughput with only two hours hands-on time.

The advantages of the BioLector microbioreactors for microbial cultivation:

  • Up to 48 simultaneous fermentations in a single microtiter plate
  • Comprehensive online monitoring of biomass (broad OD range), fluorescence, DO, pH
  • NEW: Precisely controllable oxygen supply from 0-100 % O₂ thanks to gas-tight lid*
  • Suitable for fed-batch cultivations (anaerobic/aerobic), thanks to well-individual pH & feeding control*
  • Automatable for complex experiment designs (DoE), sampling and more

Boosting your cultivations in many applications:

  • Vaccine development
  • Fed-batch development*
  • pH profiling*
  • Feeding rate optimization*
  • Media screening and optimization
  • Optimization of cultivation parameters
  • Cell line and strain screening
  • Synthetic and systems biology
  • Anaerobic / aerobic / microaerophilic cultivations
  • Statistical design of experiments (DoE)
  • Growth characterization
  • High-throughput protein expression
  • Enzyme and cell activity tests
  • Functional genomics
  • Proteomic studies
  • Inhibition and toxicity tests
  • Quality control